B4 System Biology of Product Formation by Aspergillus niger
The aim of the SFB 578 (“Sonderforschungsbereich”
“collaborative research centre”) is to investigate the
production of high value-added products such as proteins including
antibodies with recombinant cells of the filamentous fungus Aspergillus
niger. In order to reach this goal it is of particular importance
to know the interactions between the product formation, growth conditions
and morphology of the fungus. Many parameters like carbons source,
temperature, oxygen partial pressure and pH-value reveal a direct
influence on the growth of Aspergillus niger and consequently
on the product yield. To obtain an optimization of the production
process it is necessary to gain a better understanding of the molecular
and cell biology of the microorganism and of the interactions between
growth conditions, cell morphology and productiveness.
In the project B4 the investigations
will be carried out with an approach in systems biology taking into
account the transcription, the translation, and the metabolic activity
in order to determine the crucial factors, which affect the production
of proteins with Aspergillus niger. The data obtained from
the transcriptome and proteome analysis, together with the information
derived from the 13C-labelling experiments, will complement
the study about the systems biology of Aspergillus niger.
The products originated from the labelled precursors will be followed
through the metabolic pathways by means of the mass spectrometry.
Furthermore, it will be possible via bioinformatics to customize
the gene-expression data and the metabolic studies in a theoretical
model in which the limiting steps could be recognized and strategies
for the enhancement of the productivity could be suggested.